Our lab generates multiplex assays of variant effects (MAVEs) of proteins that form amyloids in human disease. We quantify how fast each sequence variant in a library is able to form amyloid aggregates. We recently generated a data set reporting on the mutational impact of all possible amino acid changes in amyloid beta, the peptide that deposits in plaques in all patients of Alzheimer’s disease. Our data accurately classifies all known familial Alzheimer’s Disease variants in amyloid-beta. We have generated data on many other amyloid sequences and would like to test whether variants that we classify as prone to form amyloids are found int he population and if they are considered benign or associated to any form of disease.
